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Primary antibodies were detected with anti-rabbit HRP-conjugated polymer and 3,3-diaminobenzidine substrate, followed by counterstaining with haematoxylin, all of which were provided in the Leica BOND Polymer Refine Detection System (DS9800)
The process must be performed carefully because peptides can be sensitive to: Temperature changes Mechanical agitation pH levels Microbial contamination Repeated freeze-thaw cycles Equipment Needed for Safe Peptide Reconstitution Lyophilised peptide vial Sterile bacteriostatic water or sterile water for injection Alcohol swabs Sterile syringe and needle Protective gloves Sterile mixing vial (optional) Labels and marker Refrigerator or laboratory freezer for storage Step-by-Step Guide to Reconstituting Peptides 1
Animals were exposed to cigarette smoke 5 days per week for a total of 6 weeks with four cigarettes given intermittently throughout the day
Phase 2 used subcutaneous 2.4 mg once weekly matching the GLP-3 dosing rhythm
Yano, K., Brown, L
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