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These effects complement the appetite-suppressing and insulin-sensitising actions of GLP-1 and GIP, creating a comprehensive metabolic effect
All 4 RCTs were multicentered (mean number of clinical sites 50) and multinational (most were done in US and Europe)
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End of study sample collection At the end of the study, at experimental day 140, an aliquot of 25 g of excreta sample was collected from each cage and freeze-dried (James Hutton Institute, Aberdeen, UK)
RINm5F cells were treated with indicated chemicals for 17 hr, and then the cells were trypsinized and washed cells twice with cold BioLegends cell staining buffer, and then the cells were resuspended in Annexin V binding buffer at a concentration of 1 10 7 cells/ml
