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Therefore, an increased supply of Met may increase antioxidant production, potentially aiding in the relief of oxidative stress and inflammation triggered by the production of reactive oxygen metabolites ( Conversely, Hcy has the capacity for remethylation to regenerate methionine, which relies on the folate cycle and vitamin B 12 ( Figure 1 ) ( 2 -THF), which is subsequently reduced to 5-methyltetrahydrofolate (5-CH 3 -THF) (Preynat et al., 2009)

Several isozymes glutathione peroxidase are encoded by different genes, which vary in cellular location and substrate specificity

To maintain continuous antioxidant defenses, the cell must recycle this oxidized molecule

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As a positive control for the impact of storage temperature, spontaneous oxidation of free GSH, and enzymatic processes, we stored an aliquot of non-deproteinized EDTA-treated plasma at 4 o C