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Western blotting analysis and enzyme-linked immunosorbent assay (ELISA) assay Whole liver lysates were prepared by sonication in HEPES lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 0.1% Tween 20, 10% glycerol, 0.1 mM Na orthovanadate, 1 mM NaF, 10 mM -glycerophosphate) containing protease inhibitor cocktail (EDTA-free Complete Mini, Roche, Meylan, France)

BMJ 379 , e071380 (2022)

By prolonging the duration of SA -induced disease resistance in plants, the nanosystem enhanced various plant defense mechanisms, including reinforcement of physical barriers (lignin and suberin deposition), upregulation of antioxidant defense enzymes (POD, SOD, and CAT), the activation of defense-related genes ( OsPR1a , OsWRKY45 , OsAOS2 , and OsJAMYB ), and the stimulation of chitinase synthesis

Additionally, it has anti - inflammatory properties that help reduce inflammation and redness associated with wounds