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Fluorescent staining for casepase3/7 was done using CellEvent TM Caspase-3/7 Green Detection Reagent Kit (Thermo Fisher Scientific)
After setting 0.030.5 mg/mL concentration of glutathione solution mixed with AgNPs and reacted, an appropriate amount of the solution to be tested was taken and added dropwise to the calcium fluoride window sheet, which was dried in an oven and then removed and fixed in a liquid cell for infrared spectroscopy testing
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Ghrelin acts through a third route, the ghrelin/growth hormone secretagogue receptor, written GHS-R1a receptor in most papers, and that is where ipamorelin binds
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