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Membranes were incubated with the following primary antibodies, ENaC (StressMarq Biosciences Cat# SPC-403, RRID: AB_10640131), Nedd4-2(Abcam Cat# ab245522), p-Nedd4-2(Cell Signaling Technology Cat# 12146, RRID: AB_2797829), GLP-1R (1:200, Bioss Cat# bs-1559R, RRID: AB_10857357), 14-3-3 protein (Cell Signaling Technology Cat# 7413, RRID: AB_10950820) and -actin (Cell Signaling Technology Cat# 4967, RRID: AB_330288) during overnight
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