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Protein concentrations were determined using a BCA protein assay (Thermo Fisher Scientific) and 60 g of protein/lane were separated by SDSPAGE using e-PAGEL (ATTO Co., Tokyo, Japan) and were electro transferred onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories Inc., Hercules, CA) for 30 min at 25 V
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Protein needs vary tremendously depending on age, body size, and physical activity levels
Losing 20 pounds could take several months, depending on your starting weight, diet, exercise, and other individual factors
Diabetes Care, 43(5), 10851093
