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The cells were isolated by centrifugation and allowed to recover in 5 mL of YPD broth at 30 C in a rotary shaker for 5 h prior to selective growth
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226227, Aug
Thus, the advantage of this limited quantity option is its limited duration, which is a trade-off in deciding whether the one-vial option is favorable
Maier JA, Martinez C, Kasavajhala K, Wickstrom L, Hauser KE, Simmerling C (2015) ff14SB: Improving the accuracy of protein side chain and backbone parameters from ff99SB
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