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The GLP-1 family acts heavily on the gut-brain axis through slowed digestion and reduced hunger signalling

The incubation mixture contained 0.9 mL of 65 mM phosphate buffer (pH7.8), 0.1 mL of 10 mM hydroxylamine hydrochloride, and 1 mL of the supernatant

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For hormone and metabolite analyses, animals were fasted at 0800 h, and trunk blood was routinely collected 4 h later at 1200 h by decapitation without anesthesia, and plasma was separated from red cells by centrifugation (1500 g 4 C 15 min) and stored at 80 C