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After synthesis, each peptide component undergoes HPLC purification to confirm chromatographic purity and mass spectrometry analysis for precise molecular weight verification
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Immunoblotting investigations into additional markers involved in ECSC isk under differentiation conditions (ECSC medium removed of growth factors) showed that the levels of all stemness genes (SOX2, NANOG and ALDH1) decreased over time, while E-cadherin and Vimentin apparently increased after long-term culturing (Supplementary Fig
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FOXO4-DRI was designed to disrupt this interaction, allowing p53 to activate programmed cell death in senescent cells during laboratory studies
