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Stopping treatment may allow gradual return to pre-treatment patterns
The synergy between medication and lifestyle changes operates through multiple channels
Lentiviruses were generated by co-transfection of each shRNA construct with envelope plus packaging vectors (pMD2.G, Addgene #12260 and psPAX2, Addgene #12259) and viral supernatants purified by ultracentrifugation onto 20% sucrose gradients
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