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After centrifuged and washed with PBS three times, the cell pellet was resuspended and cultured in Dulbeccos Modified Eagles Medium (DMEM) containing 10% FBS and cultured at 37 C in a humidified atmosphere with 5% CO 2

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DPP-4 has two mechanisms of action: first as a membrane spanning protein, where it binds and activates adenosine deaminase complexing protein and transfers intracellular signals via dimerization

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