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Inject slowly into the peptide vial: Direct the stream of liquid gently down the inside wall of the vial rather than directly onto the lyophilised powder, to minimise foaming and potential denaturation
& Sattler, M
Behavioral measurements should primarily include the percentage of time spent in the open quadrant, the percentage of entries into the open quadrant, the number of times the animal peeks down from the edge of the platform while in the open or closed quadrant, and the number of stretch-attend postures from the closed quadrant to the open quadrant
Insomnia is a difficulty falling asleep, staying asleep, or waking up early, and being unable to return to sleep
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