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The sections were then washed three times in TNT buffer (0.1 M TrisHCl, 0.15 M NaCl, 0.05% Tween20, pH 7.5) for 5 min, and treated with secondary antibodies

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Participants with knee osteoarthritis experienced a 73.1% reduction in WOMAC pain scores, while those with moderate-to-severe obstructive sleep apnea achieved a 60.6% reduction in apnea-hypopnea index, corresponding to a decrease of 36.1 breathing interruption events per hour