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The chromatographic system used a binary solvent (water and methanol) system delivered as a gradient

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Heterologous expression and sample preparation Recombinant C-terminally LEH 6 -tagged wild-type and mutant roGFP2, N-terminally MRGSH 6 GS-tagged PfGrx variants, and N-terminally MAH 6 VGT-tagged roGFP2 WT -PfGrx WT , roGFP2 WT -PfGrx C88S , and roGFP2 WT -PfGrx C32S/C88S were produced in E

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