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Injection Method: Give the solution via subcutaneous or intramuscular injection as instructed by a licensed veterinarian

This experiment was analyzed by Guangzhou Huayin Health Medical Group Co., Ltd

Novel role of 4-hydroxy-2-nonenal in AIFm2-mediated mitochondrial stress signaling

Recent developments in CuAAC have shifted towards endogenous Cu catalysts, which catalyze chemical reactions without adding exogenous Cu, mitigating the risk of perturbing copper homeostasis and toxicity against normal tissues 20,21,22

Immunoblot assays Tissue samples were crushed on dry ice to obtain homogenous aliquots and then lysed in RIPA buffer (Thermo Scientific #89900) containing Halt protease & phosphatase inhibitor (Thermo Scientific #1861280)