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Quality and quantification of total bacterial mRNA extracted was assessed using the Experion system (Experion RNA Standard Sense Kit, Bio-Rad)
It is a synthetic pentadecapeptide, a chain of 15 amino acids, derived from a protective protein found naturally in human gastric juice
The qPCR protocol consisted of initial denaturation at 95 C for 30 s, 40 cycles of amplification at 95 C for 5 s, 60 C for 30 s, and a final melting curve stage
The higher bioavailability translates to more predictable therapeutic exposure
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