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This 4.2V limit is fundamental chemistry and safety: Electrochemical Stability: At voltages significantly above 4.2V (e.g., 4.3V+), the cathode material (LiCoO or similar) becomes highly unstable

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Then, 20 g of protein was separated on a 10% SDSPAGE gel by electrophoresis, followed by transfer to a polyvinylidene difluoride membrane (Pall, BSP0161)