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For example, NMR-titration experiments of non-glutathionylated poplar GrxC4 or ScGrx8 with GSH required high glutathione concentrations to detect chemical shifts 47,48 (and titrations of active site serine mutants with GSSG cannot discriminate between both glutathionyl moieties)

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The activity of enzymes involved in its synthesis and degradation, such as choline kinase and phospholipases, is subject to various cellular signals, including nutrient availability and hormonal regulation