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doi: 10.1016/j.chembiol.2019.01.008
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Cell migration is often the rate-limiting step in tissue repair
For these applications: Reconstitute in sterile water or PBS Use immediately (within 46 hours) Prepare fresh solutions for each experiment For Mass Spectrometry or Analytical HPLC: Reconstitute in sterile water or a compatible organic solvent (e.g., acetonitrile, methanol) Avoid PBS (salt interferes with MS ionisation) Avoid BAC water (benzyl alcohol may appear as an artifact in chromatograms) For Animal Research (if applicable): Consult institutional veterinary protocols Some protocols require sterile water or PBS specifically Document the rationale for solvent choice in your IACUC or ethics application For Long-Term Studies: If a study requires peptide administration over multiple days: Reconstitute in BAC water and store at 28 C Verify stability by HPLC at the beginning and end of the study Consider aliquoting into single-day vials to minimise contamination risk Storage of Unused Solvent: BAC water: Store at room temperature (1530 C), protected from light
