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The protein samples (12 L) and Spectra Multicolor Broad Range Protein Ladder (7.5 L) with 10260 kDa range (Thermo Fisher Scientific) were loaded onto the gel, and electrophoresis was run at 200 V for 22 min in 1 Bolt MES SDS Running Buffer (Invitrogen)
USA 78 , 63726375 (1981)
Ensure you understand why both medications are necessary, what outcomes are expected, and whether alternative treatment approaches might be suitable
But the risk to begin with wasnt very large
As control of HA2X formation, 79 DIV cultured neurons were treated with doxorubicine (0.5 M) for 24 h, fixed in 4% PFA in PBS and analyzed in immunofluorescence as described above
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