BPC-157 is a synthetic 15-amino-acid peptide based on a partial sequence of Body Protection Compound, a protein originally isolated from human gastric juice
FITC-agonist was added for 30 min at 37 C to induce internalization, then removed and washed with cold HBSS to arrest further endocytosis, and cells treated with 500 mM ice-cold sodium 2-sulfanylethanesulfonate (MesNa), a membrane-impermeable reducing agent, in alkaline TNE buffer (100 mM NaCl, 50 mM Tris-HCl, pH 8.6) to cleave BG-SS-biotin specifically from cell surface receptors
10.1111/j.1471-4159.1985.tb12878.x J
Patient population stratification: which patients will benefit most (frail older, endocrine decline, metabolic syndrome)
Understanding this distinction helps patients and clinicians address fatigue appropriately, whether through medication adjustment, nutritional support, or investigation of alternative causes