Este artculo cumple con un propsito informativo y educativo, por lo que de ninguna manera sustituye la opinin de un mdico
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pH 7.3), then washed in PBS containing 15% (w/v) sucrose and 0.05% (w/v) azide for 1 h, before snap freezing in embedding medium (Tissue-Tek OCT compound, Sakura Finetek, Torrance, California, USA)