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Mechanistic sampling of intestinal tissue during this trial would best validate the reparative hypothesis if analyses include tight junction protein expression (e.g., claudins, occludin, zonula occludens-1), histologic healing assessments using validated indices (e.g., Geboes score, Robarts Histopathology Index), and intestinal permeability assays such as the lactulose-mannitol ratio
Natalie Ragson, who spearheaded this research, acknowledges that more work needs to occur in order to establish clear guidelines for dosage, frequency, and treatment duration when using Acetyl -L-Choline for improving depression symptoms
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The standard dosage is 20-40 mg/kg of body weight for 3-6 months once or twice in a day and best if taken at night 2-3 hours after the last meal for better absorption
Promising, but not definitive
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