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Library construction and sequencing The PCR products were confirmed through 2% agarose gel electrophoresis and subsequently purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA), following the manufacturers guidelines

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% Of patients waited 5 years or more for diagnosis % Of patients were initially misdiagnosed % Of patients are unhappy with their treatment Upcoming Events Become a Member of the Pernicious Anaemia Society With your help we can continue to do our valuable work, supporting those that need it and campaigning to secure future improvements in detection and treatment of this illness