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It occurs when GHK-Cu is overused, typically from daily high-concentration topical application without cycling

GHK-Cu stimulates NGF, NT-3, and NT-4 production and increases Schwann cell proliferation and nerve fiber regeneration in collagen tube models

Notably, G/D-CuP exerted anti-inflammatory and antioxidative properties, promoting angiogenesis, fibroblast proliferation, and migration to accelerate wound healing without the need for additional bioactive substances

GHK-Cu occurs naturally in human blood plasma, declining from approximately 200 ng/mL at age 20 to just 80 ng/mL by age 60, which correlates directly with the visible signs of skin aging and laxity

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