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The mixture was stored at 20 C overnight to precipitate proteins and then centrifuged at 4000 g for 20 min
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Even then, the body has regulatory mechanisms to prevent accumulation
Due to the fact that ACTL6A did not regulate NRF2, we could reach the conclusion that ACTL6A functions in conjunction with NRF2 to regulate GCLC expression at the transcriptional level
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