Injections skip all of that
If you use only 1 mL you get 10 mg/mL and every number halves, but your 0.25 mg starting dose becomes 2.5 units, which is too small to draw accurately on a U-100 barrel

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Biochem Pharmacol 2015;93:40917
Oxidative stress is thought to be one of the underlying mechanisms involved in migraine pathogenesis [3], as reactive oxygen species can cause damage to cellular structures and cause inflammation