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The concentrations of Interleukin-1 (IL-1), Interleukin-6 (IL-6), Tumor necrosis factor-alpha (TNF-), and Monocyte chemoattractant protein-1 (MCP-1) were quantified utilizing commercial ELISA kits (ELK Biotechnology, Wuhan, China)
Rotating between these areas and varying the exact spot within each region helps prevent tissue changes and reduces localised irritation
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