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[1] Unlike the folic acid derivative leucovorin, folic acid is not clinically useful in offsetting the action of folate reductase inhibitors because it requires the enzyme dihydrofolate reductase for activation

Store the vial in the refrigerator at 36 to 46 degrees Fahrenheit (2 to 8 degrees Celsius)

Real-Time PCR To quantify mRNA levels of OA and apoptosis-related gene expression, total RNA was extracted from the cells for in vitro and cartilage for in vivo according to the manufacturers instructions (iNtRON Biotechnology, South Korea)

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Notably, SHMT2 is a mitochondrial enzyme contributory to one-carbon metabolism by converting serine and tetrahydrofolate (THF) into glycine for GSH synthesis and a one-carbon unit, 5,10-methylenetetrahydrofolate (5,10-CH 2 -THF), for dTTP synthesis 35 , respectively