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In step-d), Boc-His(Trt)-Aib-Glu(OtBu)-Gly-OH (fragment-1) was condensed with peptide obtained in step-c) in presence of a coupling agent to obtain protected Semaglutide

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Biochemistry 40 , 15671576 (2001)

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Observational cohorts have mixed findings, and confounding by weight loss and diet is common