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Then, the cells were harvested and lysed with 0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5 to afford a protein free lysate
Meanwhile, that will promote the production of excessive reactive oxygen species (ROS), change the balance of oxidation and reduction in cells and will lead to oxidative stress (Rider et al., 2009
[PubMed Central ID: PMC6082033]
n = 9 for vehicle, n = 7 for vehicle+NAC, n = 6 for GGsTop, n = 9 for GGsTop+NAC)
Metformin augments Anti-Inflammatory and chondroprotective properties of mesenchymal stem cells in experimental osteoarthritis
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