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The solute carrier family 12 member 8 (Slc12a8) has been reported as a specific NMN transporter that is responsible for the uptake of NMN and maintenance of NAD + level in the murine small intestine
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For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min
