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For IF staining, fixed with 4% PFA for 15 min, permeabilized with 0.3% Triton X-100 in PBS for 10 min, and blocked with 5% normal donkey serum for 1 h at room temperature (RT)
Due to cardiomyocytes requiring massive energy demands, they are rich in mitochondria standing out from the rest of the cell type [15, 16], which makes them very sensitive to oxidative stress damage
Molecular origin and biological effects of exercise mimetics
Studies on rodents and cells have been used to suggest that BPC-157 might help heal tissues like tendons, joints, nerves, the intestinal tract, stomach and skin, but these havent been supported by studies in humans