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Lipoic acid might be able to increase glutathione synthesis in aged rats by up-regulating the expression of -glutamylcysteine ligase (-GCL), the rate-limiting enzyme in glutathione synthesis (31), and by increasing cellular uptake of cysteine, an amino acid required for glutathione synthesis (32)

The agitation breaks the weak bonds holding the protein in its active shape

10.1186/s13287-020-01691-x Stem Cell Res