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After drying the samples by speed-vac at 60 C, peptides were resuspended in 100 mM EPPS, 10 % acetonitrile (ACN), pH 8.2
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PREG then acts as an allosteric negative modulator of CB1 receptor in an autocrine-paracrine loop in the brain acting to ameliorate cannabis intoxication
It is well known that HFD-induced obesity promotes low-grade hypothalamic inflammation, causing deregulation of central mechanisms that control energy homeostasis 59 , and both physical exercise and some pharmacotherapies have been shown to improve systemic metabolism by attenuating hypothalamic inflammation 60,61
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