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Isolated perfusion with 1.0 g/kg of the cytotoxic xenobiotic 5-FU or hyperthermia of 41 degrees C 10 min resulted in 90% to 100% mortality in rats, with extensive, patchy necrosis, and infarction on histologic examination (Miyazaki et al
Mizuno S, Yoshikawa N, Seki M, Mikawa T, Imada Y (1988) Microbial production of cis , cis -muconic acid from benzoic acid
Suppression of pyruvate dehydrogenase kinase by Dichloroacetate in cancer and skeletal muscle cells is isoform specific and partially independent of HIF-1
The thermal cycling comprised an initial denaturation step at 95 C for 4 min, followed by 35 cycles at 95 C for 30 s, 59 C for 30 s and 72 C for 30 s, with the final extension at 72 C for 7 min