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5 ml of the resulting solution was adjusted to pH 3.5 and applied to a 20 ml Source 30S (Amersham Pharmacia Biotech) column equilibrated with 60 ml 0.42 % w/w citric acid, 40 % w/w 2-propanol, pH 3.5
Fitch expressed optimism about the study's results
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Medical disclaimer: This article is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment
In a follow up study with lower, sub-anorectic doses of GLP-1 (0.4 pmol/kg/min), glucagon (2.8 pmol/kg/min), GLP-1 + glucagon combination at the same doses, or placebo for 120 minutes we showed that there was a 13% decrease in food intake after the combination, with the individual infusions having no significant effect (67), further supporting the concept of co-agonism with these hormones
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