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These include sudden onset of severe leg pain, particularly if unilateral and associated with swelling or warmth (which could suggest deep vein thrombosis), progressive weakness or numbness in the legs, loss of bladder or bowel control, or symptoms accompanied by fever or signs of infection

gradual return of immunostaining and intensity was observed with decreasing concentrations of peptide antigen (Fig 4)

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Process vials in small groups so none sits half-reconstituted during a long run