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10 NO and its by-products, such as nitrate (NO3-), nitrite (NO2-), peroxynitrite (ONOO-), and 3-nitrotyrosine are called reactive nitrogen species (RNS)
Samples of medium were centrifuged, and supernatant was subsequently filtered over a 10 kD cut-off concentrator (Pierce, 88513) to remove proteins and debris
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For a study specifically isolating the variable of skin elasticity, our team would recommend using pure GHK-Cu
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