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If you must recap, use the one-handed scoop technique where you slide the cap onto the needle using the table surface, never holding the cap with your other hand

Calcium signaling in HEK293-GCAMP6s cells HEK293 were transduced with a lentivirus encoding the GCaMP6s calcium sensor (HEK293-GCaMP6s) and a stable cell line was selected using 5 g/mL puromycin treatment, as previously described 11

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lyophilization removes moisture to inhibit this Light: UV radiation can cause oxidative damage to aromatic and sulfur-containing residues Freeze-thaw cycling: Ice crystal formation during freezing can physically disrupt peptide structure