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136 In BRAFV600E-mutant cancers, loss of the aryl hydrocarbon receptor (AhR) reduces expression of its repressor AhRR, allowing ARNT to accumulate in the nucleus

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Centrifuge the cell suspension at 1000g for 5 min to obtain the cell pellet, which was then stored at 4 C for overnight fixation

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As the dose stabilises, sleep typically becomes more predictable." Finally, he emphasises when to seek advice: "If nightmares are frequent, disturbing or affecting daily life, its important to speak with a clinician