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Antibody-mediated inhibition of GDF15-GFRAL activity reverses cancer cachexia in mice

doi: 10.1002/med.21842 79 ZhaoNWangRZhouLZhuYGongJZhuangSM

When both HEK293 and U2OS cell lines are included, we incorporated a covariate into the design matrix to account for the origin of the cell lines

To prepare the RNAi-expressing bacteria, single colonies were used to inoculate small cultures (15 mL) before being scaled up to larger cultures in terrific broth (TB) supplemented with 100 g/mL carbenicillin