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The gene expression data quantified this

Since the great majority of circulating IGF-I is normally sequestered in IGFBP complexes, an analogue that escapes that buffering behaves very differently in any system containing binding proteins, and that is the reason the IGF 1 LR3 variant exists as a reagent at all

[1] Receptor selectivity tuning : The arrangement of the amino acid sequence is designed to determine the relative agonist activity at each of the three receptors

Target trial emulation: a framework for causal inference