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Thereafter, the pH was adjusted to between 5.0 and 6.0 with 100 L of 0.2 N NaOH and 40 L of 0.2 M NaH 2 PO 4 (pH 5.6)

The total amount of 1.5 ml contains eluate (1 ml), 3-methyl-2-benzothiazoline hydrazine (80 l), 3-(dimethylamino) benzoic acid (12.5 mM, 400 l) in phosphate buffer (0.375 M, pH 6.5) and 20 l (0.25 unit) of peroxidase was prepared

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Research indicates that although each peptide interacts with the somatotropic axis through different receptors and signaling motifs, their concurrent presence may produce layered regulatory dynamics within the organism