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An adequate volume of fresh cultures were then inoculated into a set of 250-mL flasks containing 50 mL of liquid YPD medium to keep an initial optical density at 600 nm (OD 600 ) value of 0.2

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The enzyme gradually loses activity during storage (Jang and Imlay, 2007), so assays were performed shortly after cell lysis

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In Wegovy studies, 3% of users experienced hair loss compared to 1% on placebo

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doi: 10.1210/jc.2015-2503 22 HoybyeCBruunJRichelsenBFlyvbjergAFrystykJ

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