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87, pp 9943-9947, Dec

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Sequential centrifugation steps are performed at incrementally increased speeds, for example, 300-3,000 g for cellular debris, 10,000 g for organelles, and 100,000 g for PDEVs, followed by resuspension of the pelleted vesicles in a biocompatible buffer such as PBS to minimize contaminants [78,79,80,81,82]