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Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively
Vaginal ultrasound examinations revealed that the treatment group had increased AFC, end-diastolic flow velocity, and peak systolic velocity in the ovarian arteries, indicating improved ovarian function
Acknowledgments We acknowledge the use of OpenAIs ChatGPT (version: GPT-4, model: gpt-4-turbo) for language editing and refinement of the manuscript
Product Overview BAC Water 30ml Large Bottle
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