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Injection volume was 5 l
After incubation, HiBiT Lytic detection reagent was prepared by dilution of LgBiT protein (1:100) and lytic substrate (1:50) in Lytic detection buffer (Promega, N3040)
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Risk of complications: Needles bring risks slight pain, infection risk, injection-site reactions
By comparing both analyses, we can distinguish general prescribing trends from those specific to adolescents with obesity, helping to contextualize the extent to which these medications are used in alignment with clinical guidelines
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