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The locations of the cell bodies of neurons recorded in the IML were confirmed after staining with NeuroTrace (435/455 blue or 530/615 red fluorescence, Invitrogen)

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Further structural analysis via the program AlphaFold 46 was conducted to visualize and better comprehend the interactions of the exendin 2029 sequence with TRPV1 within the broader context of GLP-1 and its derivatives (Supplementary Fig